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Studying recobination between the 1RS arms from the rye Petkus and Insave involved in the 1BL.1RS and 1AL.1RS translocations using storage protein loci as genetic markers
SUMMARY. A population of F6 recombinant inbred lines from the cross between winter common wheat lines with the wheat-rye translocations 1BL.1RS (of the Kavkaz type) and 1ÀL.1RS (of the Amigo type) B-16 × 7086 AR was produced. Using the storage protein loci Gli-R1, Gli-A1, and Gli-B1 as genetic markers, lines with recombinant 1RS (12 %) were identified and the frequency of recombination between 1RS involved in different translocations was determined as 7 %. 1RS from Amigo was demonstrated to carry a gene encoding the secalin ‘a’, which can be identified on SDS electrophoregrams under the y-subunit encoded by the Glu-D1 locus. The secalin ‘a’ was also revealed in the cultivar MV Táltos, which was shown to carry the 1BL.1RS translocation with secalin alleles of the Amigo type. The gene encoding the secalin ‘a’ was found to be allelic to the gene Sec-Nx from the rye Voronezhske SGI. The frequency of recombination between the loci Gli-R1 and Sec-N depends on the nature of the material analyzed and amounts about 10 % (the genetic distance of 10 cM) in the cross MV Táltos × CWX (the lines with different 1BL.1RS translocations). Such a distance suggests that important disease and pest resistance genes, in particular stem rust resistance genes, are within the region flanked by these loci. Because of this the secalin loci are convenient for primary screening for recombinant 1RS arms with new combinations of disease and pest resistance genes.
Key words: Triticum aestivum L., recombinant inbred lines, 1BL.1RS, 1ÀL.1RS, secalin loci, recombination, stem rust genes
E-mail: natalkozub gmail.com, sia1953 ukr.net, tolikkarelov meta.ua, hannabidnyk gmail.com, demianovanana gmail.com, sozinoksana gmail.com, cellbio cellbio.freenet.viaduk.net
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