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Introgressions from Aegilops mutica into the common wheat genome
SUMMARY. Introgression of genetic material from wild relatives to the genome of common wheat is relevant because it is a natural and inexhaustible source for the enrichment of the wheat gene pool with genes improving its adaptive potential. Hexaploid F5 lines of wheat type were developed by crossing common wheat Aurora (AABBDD) to genome substitution amphidiploid Aurotica (AABBTT), which carries diploid genome TT from wheat relative Aegilops mutica instead of common wheat DD subgenome. F1–F3 hybrids revealed limited self-fertility, which increased significantly in some plants of F4–F5 generations. During introgressive li-nes development cytological control of chromosome number was conducted for all generations, and observed chromosome numbers which varied from 33 to 46, and stabilized for most progenies at 42 chromosomes in F4 generation by selection of 40–44 chromosome plants in each generation. Introgression F4 lines originated from nine self-fertile plants of F2 generation; these lines differ from Aurora for some morphological traits, and carry alien DNA in their genomes, which was demonstrated by dot-blot hybridization with Aegilops mutica genome DNA as probe.
Key words: introgression lines, common wheat, Aegilops mutica, multiple introgressions, morphologic characters of wheat, karyotype, dot-blot hybridization
Tsitologiya i Genetika 2018, vol. 52, no. 1, pp. 28-40
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|Coded & Designed by Volodymyr Duplij||Modified 05.06.23|