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Phylogenetic analysis of ukrainian BYMV isolates from soybeans and beans
SUMMARY. Two isolates of Bean yellow mosaic virus originating from soybean (Glycine max L.) and bean (Phaseolus vulgaris L.) plants and distributed in Ukraine were examined according to their molecular characteristics. PCR amplification products were sequenced and compared with the corresponding sequences of different BYMV isolates obtained from GeneBank. The dates obtained in phylogenetic analysis showed 98 % sequence identities of isolates under study and in accordance with the species demarcation criteria and identification guidelines for potyviruses suggested the Ukrainian isolates as one strain of BYMV. The sequence showed high degree of sequence identities with other BYMV isolates/strains and shared maximum identity with BYMV strains reported from Russia, Australia and Argentina. The sequence data have been submitted to NCBI, accession numbers KT923790.1 for soybean isolate and KT923791.1 for bean isolate of BYMV.
Key words: potyvirus, isolates and sequences of Bean yellow mosaic virus, polymerase chain reaction with reverse transcription (RT-PCR)
Tsitologiya i Genetika 2017, vol. 51, no. 3, pp. 32-38
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|Coded & Designed by Volodymyr Duplij||Modified 01.06.23|