The polyphasic taxonomic analysis of the strain Bacillus sp. C6 – phytopathogenic microorganisms antagonist

Grabova A.Yu., Dragovoz I.V., Zelena L.B., Ostapchuk A.N., Avdeeva L.V.

SUMMARY. The polyphasic taxonomic analysis of the strain-antagonist of phytopathogenic bacteria and micromycetes was carried. It was found that the combination of culture-morphological, physiological and biochemical properties allowed to attribute the strain to the group Bacillus subtilis. It was shown that fatty acids of the cell walls of the strain were represented mainly by branched derivatives of iso- and anti iso- C15:0 and C17:0 fatty acids (85%) which was typical for the Bacillus amyloliquefaciens species. After molecular genetic analysis of the nucleotide sequence of the 16S rRNA gene and studying the profile of polymorphic nucleotides the strain was attributed to subspecies Bacillus amyloliquefaciens subsp. plantarum.

Key words: Bacillus sp. C6, cultural-morphological characteristics, physiological and biochemical properties, fatty acid composition, molecular genetic analysis, identification

Tsitologiya i Genetika 2016, vol. 50, no. 4, pp. 62-68

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Grabova A.Yu., Dragovoz I.V., Zelena L.B., Ostapchuk A.N., Avdeeva L.V. The polyphasic taxonomic analysis of the strain Bacillus sp. C6 – phytopathogenic microorganisms antagonist, Tsitol Genet., 2016, vol. 50, no. 4, pp. 62-68.

In "Cytology and Genetics":
G. Yu. Grabova, I. V. Dragovoz, L. B. Zelena, A. N. Ostapchuk, L. V. Avdeeva Polyphasic taxonomic analysis of Bacillus sp. strain C6—the antagonist of phytopathogenic microorganisms, Cytol Genet., 2016, vol. 50, no. 4, pp. 251–256
DOI: 10.3103/S0095452716040046