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Identification of QTLs on chromosome 1B for grain quality traits in bread wheat (Triticum aestivum L.)
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The present study was designed considering the importance of grain quality traits, genetic diversity and marker-trait association analysis in wheat. A significant amount of genetic diversity was found for various seed traits though the genotypes included in the study were found structured. The extent of polymorphism was high with a range of 2–13 alleles and average of 6.5 alleles per locus. Population structure was detected with 30 unlinked SSRs that divided the population of 92 genotypes in three sub-populations. Extensive LD extent was found on chromosome 1B with 42 SSRs specific for 1B chromosome. Marker-trait associations were determined using mixed linear model, where, population structure and kinship calculated on the basis of unlinked markers were covariated with 1B specific markers and traits data. Eight QTLs for five traits including protein, gluten contents, test weight bread and chapati making quality. Protein content, test weight, bread quality and Glu-B1 were found significantly associated with primers WMC419 (32 cM); WMC128 (30 cM), WMC419 (32 cM); WMC818 (17 cM) and WMC416 (44 cM), respectively.
Key words: association mapping, linkage disequilibrium, population structure, quality traits, wheat
Tsitologiya i Genetika 2016, vol. 50, no. 2, pp. 13-20
E-mail: muhammadsajjad ciitvehari.edu.pk
|Coded & Designed by Volodymyr Duplij||Modified 30.03.23|