Cytogenetic and molecular responses of ammonium sulphate application for tolerance to extreme temperatures in Vicia faba L.

Öney S., Tabur S., Tuna M.

Effects of ammonium sulphate [(NH₄)₂SO₄] on mitosis, cell cycle and chromosomes in Vicia faba L. seeds exposed to extreme temperatures were investigated using flowcytometric and cytogenetic analysis. Seeds germinated at high and low temperatures showed a significant decrease in mitotic index as compared to those of optimum temperature conditions. Application of 50 and 1000 µM (NH₄)₂SO₄ were successful in alleviating the negative effects of low and high temperature on mitotic activity, respectively. 50 µM (NH₄)₂SO₄ showed the most positive effect on cell cycle at the extreme temperatures. This concentration increased the cell division removing or decreasing the negative effects of temperature stress. Namely, the highest G2/M and S phase percentages under stress conditions were obtained with application of 50 µM (NH₄)₂SO₄. Chromosomal aberrations were not observed in cells of seeds germinated in distilled water and also at any temperatures. However, the frequency of chromosomal aberrations increased significantly by increasing (NH₄)₂SO₄ concentration. The highest aberration frequency in all temperature degree tested was found at 1000 µM (NH₄)₂SO₄ concentration.

Key words: cell cycle progression, chemical fertilizers, chromosomal abnormalities, flowcytometry, mitotic activity, temperature stress

Tsitologiya i Genetika 2015, vol. 49, no. 5, pp. 62-72

• Süleyman Demirel University Arts and Sciences Faculty, Department of Biology, Isparta, Turkey
• Namık Kemal University, Agriculture Faculty, Department of Field Crops, Tekirdağ, Turkey

E-mail: taburs gmail.com

Öney S., Tabur S., Tuna M. Cytogenetic and molecular responses of ammonium sulphate application for tolerance to extreme temperatures in Vicia faba L., Tsitol Genet., 2015, vol. 49, no. 5, pp. 62-72.

In "Cytology and Genetics":
S. Öney, S. Tabur, M. Tuna Cytogenetic and molecular responses of ammonium sulphate application for tolerance to extreme temperatures in Vicia faba L., Cytol Genet., 2015, vol. 49, no. 5, pp. 328-337
DOI: 10.3103/S0095452715050072