Obtaining of the transgenic Helianthus tuberosus L. plants, callus and «hairy» root cultures able to express the recombinant human interferon alpha-2b gene

Maistrenko O.M., Luchakivska Yu.S., Zholobak N.M., Spivak M.Ya., Kuchuk M.V.

This work is the first to our knowledge to describe the successful attempt of Agrobacterium rhizogenes-mediated transformation of topinambour in order to obtain the transgenic H. tuberosus plants, сallus and «hairy» root cultures. The plasmid vectors contained the sequence of interferon gene fused with Nicotiana plumbagenifolia L. calreticulin apoplast targeting signal driven by 35S CaMV promoter or root-specific Mll promoter. Nearly 75 % isolated Ri-root lines and callus cultures were proved (by PCR analysis) to contain HuINFα-2b transgene. We also managed to obtain H. tuberosus transgenic plants through somatic embryogenesis on the transgenic «hairy» root culture. The obtained transgenic H. tuberosus cultures exhibited high-level antiviral activity that ranged from 2000 to 54500 IU/g FW that makes this crop considered a promising source of recombinant interferon alpha 2b protein.

Key words: Helianthus tuberosus L., Agrobacterium rhizogenes-mediated transformation, recombinant human interferon alpha-2b, callus culture, «hairy» root culture

Tsitologiya i Genetika 2015, vol. 49, no. 5, pp. 38-44

• Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kyiv
• D. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv

E-mail: Yu.Luchakivskaya gmail.com

Maistrenko O.M., Luchakivska Yu.S., Zholobak N.M., Spivak M.Ya., Kuchuk M.V. Obtaining of the transgenic Helianthus tuberosus L. plants, callus and «hairy» root cultures able to express the recombinant human interferon alpha-2b gene, Tsitol Genet., 2015, vol. 49, no. 5, pp. 38-44.

In "Cytology and Genetics":
O. M. Maistrenko, Yu. S. Luchakivska, N. M. Zholobak, M. Ya. Spivak, M. V. Kuchuk Obtaining of the transgenic Heliantus tuberosus L. plants, callus and “hairy” root cultures able to express the recombinant human interferon alpha-2b gene, Cytol Genet., 2015, vol. 49, no. 5, pp. 308-313
DOI: 10.3103/S0095452715050060