Mspi-polymorphism in fourth intron of the growth hormone gene in chicken populations of different breeds. analysis of the causes of additional restriction pattern origin

Kulibaba R.A., Yurko P.S., Liashenko Y.V.

SUMMARY. The MspI-polymorphism in the fourth intron of the growth hormone gene in populations of White Plymouth Rock, Poltava Clay, Rhode Island Red and Borkovskaya Barvistaya chicken breeds was studied. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. It was found that the presence of «additional» phenotype (restriction pattern) is not associated with duplication of the growth hormone gene. The possibility of formation of heteroduplex DNA of two different types in the course of amplification of heterozygous samples B/C, containing the site ‘CCGG’, which leads to formation the additional DNA fragment which do not contain the site ‘CCGG’, was described. The nucleotide sequences of alleles A, B, C and «additional» fragment is described. Frequencies of alleles A, B and C in chicken population of White Plymouth Rock breed were 0,56; 0,16 and 0,28; Poltava Clay – 0,10; 0,07 and 0,83; Rhode Island Red – 0,27; 0,31 and 0,42; Borkovskaya Barvistaya – 0,75; 0,08 and 0,17 respectively.

Tsitologiya i Genetika 2015, vol. 49, no. 6, pp. 30-37

E-mail: romankx rambler.ru

Kulibaba R.A., Yurko P.S., Liashenko Y.V. Mspi-polymorphism in fourth intron of the growth hormone gene in chicken populations of different breeds. analysis of the causes of additional restriction pattern origin, Tsitol Genet., 2015, vol. 49, no. 6, pp. 30-37.