Antagonistic effects of Satureja hor­tensis essential oil against AFB₁ on human lym­phocytes in vitro

Ceker S., Agar G., Alpsoy L., Nardemir G., Kizil H.E.

Satureja hortensis L. (Lamiaceae) has been used as a folk remedy to treat various such as cramps, muscle pains, nausea, indigestion, diarrhea, and infectious diseases. In this study, the antagonistic effects of essential oil of S. hortensis (SHE) were studied against aflatoxin B₁ (AFB₁) in human lymphocytes in vitro. The analysis of the essential oil was performed by using Gas chromatography-mass spectrometry (GC-MS). Anti-genotoxic effects of the SHEs was evaluated using sister chromatid exchange (SCE), micronuclei (MN) tests against AFB₁. Also level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities used to determine the anti-oxidative effects of the SHEs. This result showed AFB₁ (5 µM) increased the frequencies of SCE, MN and the level of MDA. AFB₁ at the same concentration decreased the activities of SOD and GPx. However, different concentrations of SHE with AFB₁ decreased the frequency of SCE and MN and level of MDA and also increased the activities of SOD and GPx significantly. Especially, the 1.0, 1.5, 2.0 µL dose of SHE are more effective than other doses. The results of this experiment have clearly shown that SHE has strong anti-oxidative and antigenotoxic effects, these biological activities of SHEs can be due to its component.

Key words: Satureja hortensis, aflatoxin B₁, essential oils of Satureja

Tsitologiya i Genetika 2014, vol. 48, no. 5, pp. 65-71

• Ataturk University, Faculty of Science, Department of Biology, Erzurum, TR­25240, Turkey
• Fatih University, Faculty of Science, Department of Biology, Istanbul, Turkey
• Bayburt University, Education Faculty, Bayburt, Turkey

E-mail: lalpsoy fatih.edu.tr

Ceker S., Agar G., Alpsoy L., Nardemir G., Kizil H.E. Antagonistic effects of Satureja hor­tensis essential oil against AFB₁ on human lym­phocytes in vitro, Tsitol Genet., 2014, vol. 48, no. 5, pp. 65-71.