Molecular-genetic polymorphism of barley (Hordeum vulgare L.) cultivars detected with aflp method

Vdovychenko Zh.V., Sytnyk K.S., Stupak I.Yu., Spyrydonov V.G., Melnychuk S.D., Melnychuk M.D., Parii M.F.

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SUMMARY. Analysis of molecular-genetic polymorphism of barley varieties was performed using AFLP-method. A system for identification and differentiation of barley varieties based on AFLP-markers was developed. Results of testing for 19 varieties indicate a high differential ability of the developed system. Identification of varieties can be conducted using one of two proposed discriminatory sets of AFLP-markers. Based on the calculated genetic distances a dendrogram of phylogenetic relations between the varieties was constructed. The dendrogram revealed a separated origin of varieties of malting and feed directions.

Key words: AFLP, molecular markers, barley, variety identification

Tsitologiya i Genetika 2014, vol. 48, no. 2, pp. 60-70

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Vdovychenko Zh.V., Sytnyk K.S., Stupak I.Yu., Spyrydonov V.G., Melnychuk S.D., Melnychuk M.D., Parii M.F. Molecular-genetic polymorphism of barley (Hordeum vulgare L.) cultivars detected with aflp method, Tsitol Genet., 2014, vol. 48, no. 2, pp. 60-70.

In "Cytology and Genetics":
Zh. V. Vdovychenko, K. S. Sytnyk, I. Yu. Stupak, V. G. Spyrydonov, S. D. Melnychuk, M. D. Melnychuk, M. F. Parii Molecular-genetic polymorphism of barley varieties (Hordeum vulgare L.) detected by the AFLP-method, Cytol Genet., 2014, vol. 48, no. 2, pp. 117–126
DOI: 10.3103/S0095452714020108