Cytogenetic and immunogenetic analysis of recurrent pregnancy loss in women

Zastavna D., Sosnina K., Terpylyak O., Huleyuk N., Bezkorovayna H., Mikula M., Helner N.

Karyotyping of 366 couples couples (732 individuals) with early recurrent pregnancy losses in anamnesis revealed chromosomal anomalies in 4.09 % (30 cases) – within them 2.05 % carry reciprocal translocations, in 0.82 % – Robertsonian translocations, 0.55 % carry numerical and
structural gonosomal anomalies and in 0.27 % – marker chromosome of unknown origin. The risk of early reproductive losses in women after excluding the cytogenetic component increases three fold if SNPs 1082GG, 592CC, 819CC of IL-10 gene and IFN-γ +874AT or 874AA genotypes are present. ELISA-mediated detection of serum IL-10 and IFN-γ showed a possibly significant increase of IFN-γ in women with the history of early reproductive losses when compared to reproductively healthy women. We are proposing a complex cyto- and immunogenetic investigation in cases of early reproductive losses in women. One of the important issues of reproduction are the immunological mechanisms of pregnancy maintenance, where the disbalance in the genetically determined Th1- and Th2-cytokine levels may be one of the causes of early fetus elimination.

Key words: recurrent pregnancy loss, chromosomal anomalies, single nucleotide polymorphisms, IL-10 and IFN-γ

Tsitologiya i Genetika 2014, vol. 48, no. 4, pp. 44-50

Institute of Hereditary Pathology of National Academy of Medical Sciences of Ukraine, Lviv

E-mail: zastavna.d ihp.lviv.ua

Zastavna D., Sosnina K., Terpylyak O., Huleyuk N., Bezkorovayna H., Mikula M., Helner N. Cytogenetic and immunogenetic analysis of recurrent pregnancy loss in women, Tsitol Genet., 2014, vol. 48, no. 4, pp. 44-50.