SUMMARY. Plants regenerated from hairy roots and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with different efficiencies. The frequency of direct shoot formation from root cultures was up to 60 % in Digitalis and 3,7 % in Vinca. Addition of 1 mg/l BА, 0,1 mg/l NAA and 5 % sucrose to B5 medium increased regenerative capacity of Vinca roots up to 19,1 %. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology and short internodes.
Keywords: hairy root, plant regeneration, Vinca minor L., Digitalis purpurea L.