Change of the MGMT gene expression under influence of exogenous cytokines in human cells in vitro

SUMMARY. The influence of cytokines LIF, SCF, IL-3, EMAP II and the medication Laferobion (IFN-a2b) on MGMT gene expression in human cell cultures has been studied. It was shown that exogenous cytokines can modulate the MGMT gene expression at the protein level. EMAP II is able to increase or decrease the MGMT level, depending on the experimental conditions. Cytokines LIF, SCF, IL-3 and Laferobion decreased the MGMT expession level in human cells in vitro. Some conditions leading to the destruction of the MGMT protein complex were identified.

Keywords: culture of human cells, repair enzyme MGMT, regulation of expression, cytokines, protein dimers.