Increasing of rape plant resistance to parasitic nematode Heterodera schachtii using RNAi-technology

Tsygankova V.A., Yemets A.I., Iutinska H.O., Beljavska L.O., Galkin A.P., Blume Ya.B.

A vector for constitutive expression of antisense to the conservative region 8Н07 gene of nematode H. schachtii dsRNA is constructed and genetic transformation of rape plants is conducted by means of A. tumefaciens. Using molecular-genetic methods the presence of vector expression antinematode dsRNA in the genome of transgenic rape plants is shown. A high level of their silencing activity is confirmed both in nematodes and in infected plants. In laboratory studies for the physiological indexes the considerable increase of resistance was shown of transgene rape plants to root parasitic nematode H. schachtii.

Tsitologiya i Genetika 2013, vol. 47, no. 4, pp. 35-45

E-mail: vTsygankova ukr.net

Tsygankova V.A., Yemets A.I., Iutinska H.O., Beljavska L.O., Galkin A.P., Blume Ya.B. Increasing of rape plant resistance to parasitic nematode Heterodera schachtii using RNAi-technology, Tsitol Genet., 2013, vol. 47, no. 4, pp. 35-45.