Isolation and amplification сDNA of 8H07 gene conservative region of nematode heterodera schachtii with high relationship to its rape homolog

Tsygankova V.А., Andrusevich Ya.V., Ponomarenko S.P., Galkin A.P., Blume Ya.B.

SUMMARY. Original method of small regulatory si/miRNA isolation from plant cells was elaborated. PCR amplification of fragment cDNA 8H07 nematode Heterodera schachtii gene was carried out. Using Northern-blot method hybridization of plant si/miRNA with cDNA fragment of conservative region 8H07 gene the presence of their high homology is found out. The amplified cDNA fragment of nematode 8H07 gene in future will be used for creation recombinant gene with complementary antisense dsRNA sequence for increasing resistance of rape plants to parasitic nematodes.

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TSitologiya i Genetika
2012, vol. 46, no. 6, 3-11

Cytology and Genetics
2012, vol. 46, no. 6, 335-341,
doi: 10.3103/S0095452712060114

Isolation and amplification сDNA of 8H07 gene conservative region of nematode heterodera schachtii with high relationship to its rape homolog

Tsygankova V.А., Andrusevich Ya.V., Ponomarenko S.P., Galkin A.P., Blume Ya.B.

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