Possible pathways of heat resistance induction in plant cells by exogenous nitrogen oxide

Karpets Yu.V., Kolupaev Yu.E., Yastreb T.O., Dmitriev O.P.

SUMMARY. The mechanisms of influence of exogenous nitrogen oxide (NO) on heat resistance of wheat coleoptiles have been studied. The treatment of plant cells with nitrogen oxide donor (sodium nitroprusside) resulted in the increase of superoxide anion-radical (О^2•–) generation already after 10 minutes. The inhibitor of protein biosynthesis cycloheximide did not inhibit the О^2•– generation by coleoptiles caused with the NO donor whereas the inhibitor of phosphatidic acid formation (butanol-1) partially inhibited it. The treatment of coleoptiles with the calcium ionophore (А23187) or activator of inositol cycle (inositol) compensated the suppression of butanol-1 effect on NO-dependent О^2•– formation. Butanol-1 has also leveled the induction of coleoptiles heat resistance caused by the NO donor, whereas calcium ionophore and inositol almost completely removed the butanol-1 effect. The possible mechanisms of participation of reactive oxygen species, phosphatidic acid and calcium ions in the realization of NO physiological effects are discussed.

Key words: Triticum aestivum L., nitrogen oxide (NO), reactive oxygen species, NADPH oxidase, phosphatidic acid, calcium, heat resistance

Tsitologiya i Genetika 2012, vol. 46, no. 6, pp. 28-35

E-mail: plant_biology mail.ru, dmyt voliacable.com

Karpets Yu.V., Kolupaev Yu.E., Yastreb T.O., Dmitriev O.P. Possible pathways of heat resistance induction in plant cells by exogenous nitrogen oxide, Tsitol Genet., 2012, vol. 46, no. 6, pp. 28-35.

In "Cytology and Genetics":
Yu. V. Karpets, Yu. E. Kolupaev, T. O. Yastreb, O. P. Dmitriev Possible pathways of heat resistance induction in plant cells by exogenous nitrogen oxide, Cytol Genet., 2012, vol. 46, no. 6, pp. 354-359
DOI: 10.3103/S0095452712060059