Features of lettuce transgenic plants with ifn­α2b gene regenerated after Agrobacterium rhizogenes­mediated transformation

Matvieieva N.A., Shahovsky А.М., Kuchuk М.V.

SUMMARY. «Hairy» roots of lettuce Lactuca sativa and regenerated plants with interferon-α2b gene had been obtained via Agrobacterium rhizogenes-mediated transformation. According to the results of PCR and rt-PCR analyses the studied plants had ifn-α2b gene. The regenerated plants differed from the plants of wild type by elongated internodes, early flower-bearing stem formation and purple coloration of leaves in artificial illumination conditions.

Key words: Lactuca sativa, Agrobacterium rhizogenes, transformation, ifn-α2b gene

Tsitologiya i Genetika 2012, vol. 46, no. 3, pp. 27-32

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Matvieieva N.A., Shahovsky А.М., Kuchuk М.V. Features of lettuce transgenic plants with ifn­α2b gene regenerated after Agrobacterium rhizogenes­mediated transformation, Tsitol Genet., 2012, vol. 46, no. 3, pp. 27-32.

In "Cytology and Genetics":
N. A. Matvieieva, A. M. Shakhovskij, M. V. Kuchuk Features of lettuce transgenic plants with the ifn-α2b gene regenerated after Agrobacterium rhizogenes-mediated transformation, Cytol Genet., 2012, vol. 46, no. 3, pp. 150-154
DOI: 10.3103/S0095452712030073