Obtaining of hairy­root, callus and suspension carrot culture (Daucus carota L.) able to accumulate human interferon alpha­2b

Luchakivskaya Y.S., Olevinskaya Z.M., Kishchenko Y.M., Spivak N.Y., Kuchuk N.V.

Here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of Nantskaya and Perfektzya variety with the highest level of recombinant human interferon α-2b accumulation exhibited the highest level of plant protein extract antiviral activity (up to 12.8 · 10³ IU/mg TSP). The antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from parent organisms. However, the antiviral activity level of suspension culture extracts (up to 4.42 · 10³ IU/mg TSP) and Ri-root ones (up to 4.42 · 10³ IU/mg TSP) appeared to be comparable to analogical data of antiviral activity of transgenic carrot leaf extracts, this way the described cultures could be possibly used for comparatively speedy obtaining of recombinant therapeutic protein for curing and preventing of virus diseases.

Tsitologiya i Genetika 2012, vol. 46, no. 1, pp. 18-26

E-mail: yu.luchakivskaya gmail.com

Luchakivskaya Y.S., Olevinskaya Z.M., Kishchenko Y.M., Spivak N.Y., Kuchuk N.V. Obtaining of hairy­root, callus and suspension carrot culture (Daucus carota L.) able to accumulate human interferon alpha­2b, Tsitol Genet., 2012, vol. 46, no. 1, pp. 18-26.