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Molecular-genetic analysis of the breeding bread wheat lines with starch of amylopectin type

Semenjuk I.V., Chebotar S.V., Rybalka A.I., Sivolap Yu.M.

 


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PCR-analysis with primers to Wx-loci of T. aestivum L. genome lets us run the controlled selection of genotypes with null-alleles of Wx-genes while creating bread wheat lines with amylopectin type of starch. Microsatellite analysis of selected individual plants which were the carries of three null-alleles of Wx-genes (Wx-A1b, Wx-B1b, Wx-D1b) and the cluster analysis in the complex allowed us to pick out four genotypes, which were very close related genetically to the parent form variety Kuyalnik.

Key words: amylose, starch of amylopectin type, microsatellite, Wx-genes

Tsitologiya i Genetika 2011, vol. 45, no. 5, pp. 17-22

E-mail: ivpetrova-15 mail.ru

Semenjuk I.V., Chebotar S.V., Rybalka A.I., Sivolap Yu.M. Molecular-genetic analysis of the breeding bread wheat lines with starch of amylopectin type, Tsitol Genet., 2011, vol. 45, no. 5, pp. 17-22.

In "Cytology and Genetics":
I. V. Semenyuk, S. V. Chebotar’, A. I. Rybalka, Yu. M. Sivolap Molecular and genetic analysis of soft wheat selection lines with starch of the amylopectin type, Cytol Genet., 2011, vol. 45, no. 5, pp. 282-287
DOI: 10.3103/S0095452711050112


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