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Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots
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Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon a2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants.
Key words: Cichorium intybus L., Agrobacterium rhizogenes, genetic transformation, genes for tuberculosis antigenes ESAT6 and Ag85B or human interferon α2b
Tsitologiya i Genetika 2011, vol. 45, no. 5, pp. 11-16
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