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Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots

Matvieieva N.A., Kishchenko O.M., Shakhovsky A.M., Potrochov A.O., Kuchuk M.V.

 


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Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon a2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants.

Key words: Cichorium intybus L., Agrobacterium rhizogenes, genetic transformation, genes for tuberculosis antigenes ESAT6 and Ag85B or human interferon α2b

Tsitologiya i Genetika 2011, vol. 45, no. 5, pp. 11-16

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Matvieieva N.A., Kishchenko O.M., Shakhovsky A.M., Potrochov A.O., Kuchuk M.V. Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots, Tsitol Genet., 2011, vol. 45, no. 5, pp. 11-16.

In "Cytology and Genetics":
N. A. Matvieieva, O. M. Kishchenko, A. O. Potrochov, A. M. Shakhovsky, M. V. Kuchuk Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi, Cytol Genet., 2011, vol. 45, no. 5, pp. 277-281
DOI: 10.3103/S0095452711050082

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