Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression

Sindarovska Y.R., Gerasymenko I.M., Sheludko Y.V., Olevinskaya Z.M., Spivak N.Y., Kuchuk N.V.

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Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 · 10³ IU/g fresh weight (FW) with an average of 2.1 ± 0.8 · 10³ IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b.

Tsitologiya i Genetika 2010, vol. 44, no. 5, pp. 60-64

• Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kiev 03680, Ukraine
• Institute of Microbiology and Virology NAS of Ukraine, Kiev

E-mail: ysheludko ukr.net

Sindarovska Y.R., Gerasymenko I.M., Sheludko Y.V., Olevinskaya Z.M., Spivak N.Y., Kuchuk N.V. Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression, Tsitol Genet., 2010, vol. 44, no. 5, pp. 60-64.