RAPD-analysis of soybean cell line with cross-resistance to tungstem and vanadium oxyanions

The effect of vanadium oxyanions on genome of soybean (Glycine max L., Merr.) in tungsten-resistant cell line was studied. The line is resistant to V⁵⁺-oxyanions. RAPD-amplicons with identical length are differentially synthesized from DNAs of tungsten-resistant cell line as well as of the initial culture was shown by using the lethal dose of oxyanions W⁶⁺ and then V⁵⁺. The presence of instable loci in soybean genome under different stressors is discussed.

Keywords: soybean (Glycine max L., Merr.), RAPD-PCR, tungsten/ vanadium-resistant, cell selection