Molecular polymorphism of cellular lines of wheat resistant to culture filtrate Gaeumannomyces graminis var. tritici, and plant-regenerants from them

Bavol A.V., Dubrovna O.V.

DNA polymorphism of wheat cellular lines resistant to culture filtrate of G. graminis var. tritici and regenerated plants has been investigated using ISSR-analysis. Specific changes in DNA sequence were revealed in resistant calluses. It was established, that all resistant cellular lines differed by the presence of specific amplicons of 2347 bp (primer 5’-TCTCTCTCTCTCTCTCG-3’) and 1745 bp (primer 5’-AGAGAGAGAGAGAGAGTC-3’), as well as the absence of the amplicon of 1108 bp (primer 5’-ACACACACACACACACC-3’) from the initial callus and the callus not exposed to the action of selective factor. These changes have been also revealed in R₀ and R₁ plants.

Key words: Triticum aestivum L., cellular selection, culture filtrate of Gaeumannomyces graminis var. tritici, plant regeneration, ISSR-analyse

Tsitologiya i Genetika 2009, vol. 43, no. 5, pp. 28-34

E-mail: bavol1 rambler.ru

Bavol A.V., Dubrovna O.V. Molecular polymorphism of cellular lines of wheat resistant to culture filtrate Gaeumannomyces graminis var. tritici, and plant-regenerants from them, Tsitol Genet., 2009, vol. 43, no. 5, pp. 28-34.