DNA polymorphism of wheat cellular lines resistant to culture filtrate of G. graminis var. tritici and regenerated plants has been investigated using ISSR-analysis. Specific changes in DNA sequence were revealed in resistant calluses. It was established, that all resistant cellular lines differed by the presence of specific amplicons of 2347 bp (primer 5’-TCTCTCTCTCTCTCTCG-3’) and 1745 bp (primer 5’-AGAGAGAGAGAGAGAGTC-3’), as well as the absence of the amplicon of 1108 bp (primer 5’-ACACACACACACACACC-3’) from the initial callus and the callus not exposed to the action of selective factor. These changes have been also revealed in R0 and R1 plants.
Keywords: Triticum aestivum L., cellular selection, culture filtrate of Gaeumannomyces graminis var. tritici, plant regeneration, ISSR-analyse
