TSitologiya i Genetika 2009, vol. 43, no. 2, 27-32
Cytology and Genetics , vol. , no. , , doi: https://www.doi.org/

Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes of bacterial antigenes from Mycobacterium tuberculosis

Matvieieva N.A., Vasylenko M.Y., Shahovsky A.I., Kuchuk N.V.

Transgenic plants of lettuce Lactuca sativa L. cv. Eralash, Sniezinka, Rubinovoje kruzevo with genes coding synthesis of tuberculosis antigenes have been obtained by Agrobacterium-mediated transformation. Cotyledons of in vitro seedlings were used as the initial material for transformation with plasmids pCB063 (genes ESAT6, nptII) and pCB064 (genes ESAT6:Ag85B(-TMD), nptII). PCR-analysis has shown the presence both selective and target genes in all plants analyzed. At the same time, the RT-PCR has shown that both the presence and the absence of a transcription of gene ESAT6 at a stable transcription of a gene nptII is possible.

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TSitologiya i Genetika
2009, vol. 43, no. 2, 27-32

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