A cocultivation system was developed to investigate the interactions between MCF-7 breast cancer epithelial cells and MT-4 (line human T-cell leukemia) by paracrine mechanism (no contact cocultivation). Viability and proliferation were determined in two fractions of MCF-7 cells from spheroid culture and monolayer. It has been shown that the influence of MT-4 reduced the number of cells MCF-7 in fraction of adhesive cells and did not influence on the suspension fraction. Proliferation was reduced in both fractions without MT-4. Cells viability was different in monolayer and spheroids with MT-4 and without MT-4; in monolayer it was lower, in spheroids it was higher. We think that, MT-4 by paracrine signal way stimulated proliferation MCF 7 cells, reduced adhesive to the bottom and led to MTS formation.
Keywords: Multicellular tumor spheroids (MTS, 3-D culture ), monolayer (2-D culture), MCF-7, MT-4, coculturing, proliferation, viability, adhesiveness
