Kinetics of growth 2-D and 3-D cell models under influence of microenvironment

Garmanchouk L.V., Perepelytsina O.M., Sydorenko M.V., Ostapchenko L.I.

A cocultivation system was developed to investigate the interactions between MCF-7 breast cancer epithelial cells and MT-4 (line human T-cell leukemia) by paracrine mechanism (no contact cocultivation). Viability and proliferation were determined in two fractions of MCF-7 cells from spheroid culture and monolayer. It has been shown that the influence of MT-4 reduced the number of cells MCF-7 in fraction of adhesive cells and did not influence on the suspension fraction. Proliferation was reduced in both fractions without MT-4. Cells viability was different in monolayer and spheroids with MT-4 and without MT-4; in monolayer it was lower, in spheroids it was higher. We think that, MT-4 by paracrine signal way stimulated proliferation MCF 7 cells, reduced adhesive to the bottom and led to MTS formation.

Key words: Multicellular tumor spheroids (MTS, 3-D culture ), monolayer (2-D culture), MCF-7, MT-4, coculturing, proliferation, viability, adhesiveness

Tsitologiya i Genetika 2009, vol. 43, no. 5, pp. 14-21

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Garmanchouk L.V., Perepelytsina O.M., Sydorenko M.V., Ostapchenko L.I. Kinetics of growth 2-D and 3-D cell models under influence of microenvironment, Tsitol Genet., 2009, vol. 43, no. 5, pp. 14-21.