Cytology and Genetics ,
vol. ,
no. , ,
doi: https://www.doi.org/
New model system of plastid transformation has been proposed using a wild representative of Solanaceae family – S. sinuata. Earlier obtained cybrid plants N. tabacum (+S. sinuata) were used for transformation experiments by PEG treatment of protoplasts with aadA gene that confers resistance to spectinomycin. Transformed S. sinuata plastome was transferred from N. tabacum (+S. sinuata) cybrid to S. sinuata wild type plants by somatic hybridization. Molecular analysis of nuclear and mitochondrial DNA has been performed.
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