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An analysis of the cDNA nucleotide sequence for the C-terminal fragment of the coat protein in tobacco mosaic virus isolates from tobacco growing in an ecological niche of Polesye, Ukraine

Boyko A.L., Stepaniuk S.A., Garifulin O.M.

 




wo identical strains of tobacco type TVM have been isolated in the region with 137Cs nuclear contamination with density of 12.6 Cu/km2 recombinant plasmids (pTVM9, pTVM9,5) containing cDNA of complete provirus and C-end sequence of cDNA of specific capsid protein from one of isolated viruses have been obtained. The capsule proteins of isolated strains have the higher 19.5 +/- 1.9 kDa molecular weight than standard TVM strain (17.5 kDa) as to SDS-PAAG electrophoresis data. No differences in distribution of fragments immunoactive to control antiserum have been found when using immunoblot analysis of capsid proteins of isolates and standard strain treated by tripsin. Sequencing analysis of cDNA pTVM9,5 has revealed non-conservative amino acid replacement of serine by tyrosine in position 149 for homologous region of capsid protein of standard TVM strain, which allows to suppose the mediated c86 effect of specific ecological situation on the appearance of such replacement.

Tsitologiya i Genetika 1998, vol. 32, no. 3, pp. 30-35



Boyko A.L., Stepaniuk S.A., Garifulin O.M. An analysis of the cDNA nucleotide sequence for the C-terminal fragment of the coat protein in tobacco mosaic virus isolates from tobacco growing in an ecological niche of Polesye, Ukraine, Tsitol Genet., 1998, vol. 32, no. 3, pp. 30-35.




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