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Karyotypic study of five lutjanid species using conventional and AgNORs banding techniques
The first cytogenetic comparisons of five snapper species from Thailand were presented here. Renal cell samples were taken from blacktail snapper (Lutjanus fulvus), five lined snapper (L. quinquelineatus), dory snapper (L. fulviflamma), brownstripe red snapper (L. vitta), and mangrove red snapper (L. argentimaculatus). The mitotic chromosome preparation was prepared di
rectly from kidney cells. Conventional staining and AgNOR banding techniques were applied to stain the chromosomes. The results exhibited that all five snapper species have the diploid chromosome numbers of 2n = 48 and the fundamental numbers (NF) of 48. The presences of large, medium, and small telocentric chromosomes were 22242, 24204, 36102, 28164 and 36102, respectively. The AgNORs banding technique provides the pair of nucleolar organizer regions (NORs) at subcentromeric region of the long arm of the respective telocentric chromosome pairs 9, 1, 3, 4 and 9. Their karyotype formulas is as follows: L. fulvus (2n = 48): Lt22+Mt24+St2, L. quinquelineatus (2n = 48): Lt24+Mt20+St4, L. fulviflamma (2n = 48): Lt36+Mt10+St2, L. vitta (2n = 48): Lt28+Mt16+St4, and L. argentimaculatus (2n = 48): Lt36+Mt10+St2.
Key words: Lutjanus, Chromosome, Karyotype, Nucleolar organizer regions
Tsitologiya i Genetika 2017, vol. 51, no. 4, pp. 81-83
- Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand
- Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Muang, Surin 32000, Thailand
- Faculty of Agriculture and Technology, Rajamangala University of Technology Issan Surin Campus, Muang, Surin 32000, Thailand
- Biology Program, Department of Science, Faculty of Science, Buriram Rajabhat University, Muang, Buriram 31000, Thailand
E-mail: tanomtong hotmail.com
Phimphan S., Supiwong W., Tanomtong A., Pinthong K., Saowakoon S., Kaewsri S. Karyotypic study of five lutjanid species using conventional and AgNORs banding techniques, Tsitol Genet., 2017, vol. 51, no. 4, pp. 81-83.