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Loss of heterozygosity of individual loci in Arabidopsis thaliana regenerants cultured with para-fluorophenylalanine

Zimina O.V., Parii M.F., Alkhimova O.G.

 




SUMMARY. Precise chromosome segregation is vital for speciation and hybrid formation. The aim of the work was to study the chromosomes behaviour and inheritance of maternal and paternal genomes in Arabidopsis regenerants de-rived from in vitro cultured cells on the medium with PFFA. The Arabidopsis thaliana model hybrid between Columbia and Landsberg erecta ecotypes was developed, which chromosomes were easy to distinguish using the 12 SSLP selected markers. Also, the influence of PFFA on the callus formation and regeneration of plants was analysed. 20 regenerated plants cultured with PFFA were derived, three of which were shown to loss the heterozygosity in six loci by DNA markers analysis. Different models are certainly required to understand how and when the mechanisms leading to proper chromosome segregation are established in species and hybrids.

Key words: Arabidopsis thaliana ecotypes, PFFA, loss of heterozygosity, SSLP markers, chromosomal loci

Tsitologiya i Genetika 2016, vol. 50, no. 5, pp. 17-24

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Zimina O.V., Parii M.F., Alkhimova O.G. Loss of heterozygosity of individual loci in Arabidopsis thaliana regenerants cultured with para-fluorophenylalanine, Tsitol Genet., 2016, vol. 50, no. 5, pp. 17-24.

In "Cytology and Genetics":
O. V. Zimina, M. F. Parii, O. G. Alkhimova Loss of heterozygosity at individual loci in Arabidopsis thaliana regenerants cultured with para-fluorophenylalanine, Cytol Genet., 2016, vol. 50, no. 5, pp. 278Ц284
DOI: 10.3103/S0095452716050157


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