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Wheat plant dna methylation pattern changes at seed chronic γ irradiation

Kravets A.P., Mousseau T.A., Litvinchuk A.V., Ostermiller Sh., Vengen G.S., Grodzinskiy D.M

 


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Alterations of DNA methylation patterns of wheat two varieties Odessa albatross and Donetsk 48 have been studied. Seeds were irradiated for 4 months with low dose rate (3 107 Gy/s). Six restriction endonucleases were used in the experiments. Primary distinction in DNA methylation patterns of the studied varieties has been demonstrated. The chronic irradiation resulted in the increase of methylation level on the sites of recognition for GluI and Sou3A1 and in the decline of this index for the sites of recognition of GlaI and HpaII. The meaningful increase of chromosome aberration levels was demonstrated at the same accumulated dose of chronic irradiation. The role of changes of DNA methylation patterns in development of radiation damage and organism protective reactions is discussed.

Tsitologiya i Genetika 2010, vol. 44, no. 5, pp. 18-22

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Kravets A.P., Mousseau T.A., Litvinchuk A.V., Ostermiller Sh., Vengen G.S., Grodzinskiy D.M Wheat plant dna methylation pattern changes at seed chronic γ irradiation, Tsitol Genet., 2010, vol. 44, no. 5, pp. 18-22.




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