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Study of T-DNA integration loci in tobacco transgenic plants

Filipenko E.A., Filipenko M.L., Deineko E.V., Shumny V.K.

 


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From the total DNA of 17 transgenic tobacco plants the DNA fragments containing T-DNA/plant DNA junctions were amplified using inverse polymerase chain reaction. Comparison of the nucleotide sequences of 34 fragments with the GENEBANK sequences revealed homology with vector sequences outside T-DNA in 10 cases and no homology with the known nucleotide sequences in most clones. The AT-content varied from 51 up to 72 % that is close to the total percentage of АТ pairs in tobacco genome. Alignment of the sequences truncated during embedding of the left and the right borders has shown that for the left border significant clusterization (10 bp region) of truncation sites was observed, and five sequences had identical sites of truncation (+ 23 Т) that showed the preferable use of this nucleotide. Nine created nucleotide sequences were homologous to the repeating sequences in tobacco genome. The percentage of homology varied from 70 up to 90 %. The identified repeats belong to different types.

Tsitologiya i Genetika 2007, vol. 41, no. 4, pp. 3-8

E-mail: deineko bionet.nsc.ru, filipenko bionet.

Filipenko E.A., Filipenko M.L., Deineko E.V., Shumny V.K. Study of T-DNA integration loci in tobacco transgenic plants, Tsitol Genet., 2007, vol. 41, no. 4, pp. 3-8.




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