Brik A.F., Kalendar R.N., Stratula O.R., Sivolap Yu.M.

IRAP and REMAP analyses of barley varieties of Odessa breeding

Cytology and Genetics 2006, v 40, number 3, 24-33

Application of polylocus biallelic systems of PCR along with monolocus SSR-analysis is very promising approach for detailed characterization, differentiation and identification of crop varieties. Microsatellite sequences and LTR retratransposon fragments are known to be the most variable in plant genome. They can be used in PCR analysis as IRAP and REMAP. Conditions of IRAP and REMAP analyses of intra- and intervariety polymorphism of the barley varieties of Odessa breeding have been elaborated. The detailed genotype formulas are represented which reflect the intravariety polimorphism and make it possible to detect the changes in variety structure in the course of seed production process.