Breast Cancer BC is the extreme most frequent cancer among women. Death from BC is around 15 % of all cancer deaths among women. BC risk factors are classified into non-modifiable risk factors and modifiable risk factors. Excessive studies on folate pathway have been done as a possible mechanism for rising cancer. The present work aimed to study genetic polymorphisms in cystathionine beta-synthase CBS (CBS 844ins68) and the cytosolic serine hydroxy-methyltransferase-1 SHMT1 (C1420T) genes and their expression in patients with breast cancer . Methodology the study included 100 diagnosed breast cancer patient and 100 healthy as a control. Conclusion of this study, results obtained indicated that; there is a significant association between CBS 844ins68 polymorphism and increased risk of breast carcinoma as There was a significant difference in the frequency of the mutant insertion allele between breast cancer patients and control subjects, Also for SHMT1 C1420T SNP, there was a significant difference in the frequency of the mutant T allele between breast cancer patients and control subjects that reflected that SHMT1 C1420T may has a protective effect against breast cancer. On The level of gene expression of CBS and SHMT1 genes, there was insignificant difference between malignant and adjacent normal tissue samples as a control.
Keywords: Breast cancer, CBS (CBS 844ins68), SHMT1 (C1420T), gene expression
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References
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