TSitologiya i Genetika 2022, vol. 56, no. 3, 76-79
Cytology and Genetics 2022, vol. 56, no. 3, 292–300, doi: https://www.doi.org/10.3103/S0095452722030094

4-Hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells

Öner Ç., Soyergin D., Özyurt A., Çolak E.

  1. Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey
  2. Maltepe University, Medical Faculty, İstanbul, Turkey
  3. Eskişehir Osmangazi University, Medical Faculty, Department of Biostatistics, Eskişehir, Turkey

4-Hydroxycoumarin is an aromatic substance which is metabolized in liver and used as a therapeutic agent for various diseases. We aimed to determine the impact of 4-Hydroxycoumarin on HepG2 cells according to their viability, proliferation, adhesion and gene expression of cellular behavior parameters. Inhibitory concentration 50 (IC50) of 4-Hydroxycoumarin was detected on HepG2 cells. After determining the optimal time and concentration, the effect of 4-Hydroxycoumarin on viability, proliferation and adhesion of HepG2 cells were observed. Gene expressions of Ki-67, MMP-2, MMP-9 and piR-823 expression were determined by using Real Time-Polymerase Chain Reaction. IC50 value of 4-Hydroxycoumarin on HepG2 cells was 5 µM at the 48th hour (p < 0.001). 5 µM at the 48th hour of 4-Hydroxycoumarin caused to decrease of proliferation (p < 0.001) and viability of HepG2 cells (p < 0.001). Viability rate were supported by hematoxylin-eosin staining. Adhesion of cells increased on 4-Hydroxycoumarin treated cells compared to control (p < 0.001). While Ki-67 gene expression of 4-Hydroxycoumarin treated group decreased (p < 0.001); upregulation of MMP-2, MMP-9 and piR-823 expressions were determined in 4-Hydroxycoumarin treated group (p < 0.001). According to the cellular and genetic perspective, 4-Hydroxycoumarin might be useful to treat hepatocellular carcinoma. High adhesion and proliferation are the main characteristics of HepG2 cells, 4-Hydroxycoumarin treatment caused to lose these functions. The genetic markers of these characteristics also supported the same result. These are first findings about the effect of 4-Hydroxycoumarin on piR-823 and genes which are key features of cellular survival mechanisms. 

Keywords: 4-Hydroxycoumarin, Matrix metalloproteinase, piRNA, piR-823, Hepatocellular carcinoma

TSitologiya i Genetika
2022, vol. 56, no. 3, 76-79

Current Issue
Cytology and Genetics
2022, vol. 56, no. 3, 292–300,
doi: 10.3103/S0095452722030094

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