SUMMARY. Seasonality affects the morphofunctional characteristics of reproductive cells, which makes the natural reproduction of dairy goats possible only in certain seasons. Cryopreservation of sperm, as part of assisted reproductive technologies, provides their flexibility, which increases the chances of increasing the number of livestock. However, it can cause changes in the morphofunctional characteristics and genetic material of sperm. Therefore, the aim of this study was to determine seasonal changes in viability, motility and DNA fragmentation level of native and cryopreserved goat sperm. The experiment was conducted using ejaculates of sexually mature male goats of Saanen breed, obtained in breeding and non-breeding seasons. To detect the effect of seasonal differences of seminal plasma on the characteristics of cryopreserved sperm, cells were cryopreserved in ejaculate and following isolation. The results of the study showed that the motility of native ejaculate sperm in the breeding season was higher than in the non-breeding season (p ≤ 0.05). Cryopreservation led to a decrease in the number of motile sperm of ejaculate and the selected fraction of sperm in the non-breeding season, and the selected fraction of cells in the breeding season (p ≤ 0.05). When comparing the viability and integrity of sperm DNA, there was a significant decrease in all groups in the non-breeding season compared to the breeding season (p ≤ 0.05). It was found that the level of DNA fragmentation of goat ejaculate sperm in the breeding season after cryopreservation did not change compared to the native sample, while in cryopreserved sperm of the selected fraction of the same season increased (p ≤ 0.05). Cryopreserved spermatozoa of the non-breeding season in the ejaculate and isolated fraction had an increased level of DNA fragmentation compared to baseline before cryopreservation. Thus, it can be concluded that the composition of semen liquid varies depending on the mating season, which affects its cryoprotective properties against spermatozoa during the ejaculate freezing. Therefore, it is recommended to collect whole ejaculate by freezing in autumn and early winter to improve the effectiveness of artificial insemination using cryopreserved sperm of male goats of Saanen breed.
Keywords: seasonality, cryopreservation, DNA fragmentation, goat spermatozoa, seminal fluid
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