TSitologiya i Genetika 2020, vol. 54, no. 5, 89-96
Cytology and Genetics 2020, vol. 54, no. 5, 449–455, doi: https://www.doi.org/10.3103/S0095452720050102

The influence of blue color low-intensity light radiation on physical and chemical properties of erythrocytes of rats blood during diabetes mellitus

Yefimenko N., Lіuta M., Karmash O., Hizhetska O., Korobov A., Sybirna N.

  1. Ivan Franko Lviv National University, St. Hrushevskogo, 4, Lviv, 79005, Ukraine
  2. V.N. Karazin Kharkiv National University, Svobody Sq., 4, Kharkiv, 61022, Ukraine

SUMMARY. The study of physical and chemical characteristics of peripheral blood erythrocytes membranes in rats with diabetes mellitus during low-intensity blue light therapy was conducted by acid hemolysis method. The using of low-intensity light therapy (LLT) led to decreasing of erythrocytes membranes resistance to acidic hemolytic in control group of animals. In diabetes conditions LLT caused the increasing of total hemolysis duration and decreasing the number of hemolysed erythrocytes compared to the indicies in non-treated diabetic animals. There was no significant hypoglycemic effect in diabetic animals during LLT treatment, but the level of glycosylated hemoglobin had tendency to decrease.

Keywords: diabetes mellitus, erythrocytes, low-intensity light therapy (LLT)

TSitologiya i Genetika
2020, vol. 54, no. 5, 89-96

Current Issue
Cytology and Genetics
2020, vol. 54, no. 5, 449–455,
doi: 10.3103/S0095452720050102

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References

1. Karandashov, V.I., Biological effects of blue light irradiation and perspectives of its application in practical medicine, Photobiol. Exp. Photomed., 2013, vol. 1, no. 2, pp. 98–106.

2. Stern, M., Broja, M., Sansone, R., Gröne, M., Skene, S.S., Liebmann, J., Suschek, C.V., Born, M., Kelm, M., and Heiss, C., Blue light exposure decreases systolic blood pressure, arterial stiffness, and improves endothelial function in humans, Eur. J. Prevent. Cardiol., 2017, vol. 25, pp. 1875–1883. https://doi.org/10.1177/2047487318800072

3. Fedorov, S.M., Bazilyuk, O.V., Korkach, Y.P., and Sagach, V.F., Magnetic-laser influence on the system of nitric oxide and contractile activity of smooth muscles of rat aorta under hypertension, Fiziol. Zh., 2012, vol. 58, no. 6, pp. 36–47. https://doi.org/10.15407/fz

4. Fiodorov, S.M., The modern concept of magnetic-laser and photomagnetic therapy and its use in neurological practice, Photobiol. Photomed., 2016, vol. 1, no. 2, pp. 38–50.

5. Serbin, M.E., Timchenko, D.S., Korobov, A.M., Laguta, T.I., and Shidlovska, O.A., Biostimulative implants and enhancement devices quality of their use, Photobiol. Photomed., 2017, vol. 1, no. 2, pp. 95–104.

6. KazemiKhoo, N. and Ansari, F., Blue or red: which intravascular laser light has more effects in diabetic patients? Lasers Medical Sci., 2014, vol. 30, no. 1. https://doi.org/10.1007/s10103-014-1672-7

7. Osinskiy, V., Pavlov, S., Tuzhanskiy, S., Kaminskiy, O., and Temchyshena, A., Laser and photonic light sources for photomedicine, Photobiol. Photomed., 2010, vol. 3, no. 4, pp. 91–97.

8. Popov, N.N., Malanchuk, S.G., Filimonova, N.I., Mishina, M.M., Korobov, A.M., and Liapunov, N.À., Integrated effect of photodiode emission and antiseptic drugs with disodium edetate on the daily biofilms of E. coli,Ukr. Biopharmaceut. J., 2014, vol. 5, no. 34, pp. 74–78.

9. Senatorova, H.S., Nikolaieva, O.V., Makieieva, N.I., Ishchenko, T.B., Riha, O.O., and Muratov, H.R., Erythropoiesis in Children in Normal and Pathological Conditions, KhNMU, 2010.

10. Sybirna, N.O., Burda, V.A., and Chajka, Ya.P., Methods of Blood System Research, Lviv: Lviv. Nats. Univ., 2006.

11. Drabkin, D.L., The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species, J. Biol. Chem., 1946, vol. 164, no. 2, pp. 703–723.

12. Shevchuk, V.H., Moroz, V.M., Belan, S.M., Hzhehotskyi, M.R., and Yoltukhivskyi, M.V., Physiology, Vinnytsia: Nova Knyha, 2015.

13. Grossweiner, L.I., Fernandez, J.M., and Bilgin, M.D., Photosensitisation of red blood cell haemolysis by photodynamic agents, Lasers Med. Sci., 1998, vol. 13, pp. 42–54.

14. Eberlein-König, B., Placzek, M., and Przybilla, B., Phototoxic lysis of erythrocytes from humans is reduced after oral intake of ascorbic acid and alpha-tocopherol, Photodermatol. Photoimmunol. Photomed., 1997, vol. 13, no. 5–6, pp. 173–177.

15. Masalunga, C., Cruz, M., Porter, B., Roseff, S., Chui, B., and Mainali, E., Increased hemolysis from saline pre-washing RBCs or centrifugal pumps in neonatal ECMO, J. Perinatol., 2007, vol. 27, no. 6, pp. 380–384.

16. Kriventsev, Yu.A., Biochemistry: Structure and Role of Hemoglobin Profile Proteins, Moscow: Yurait, 2018.

17. Schmidt, A., Refaai, M., Kirkley, S., and Blumberg, N., Proven and potential clinical benefits of washing red blood cells before transfusion: current perspectives, Int. J. Clin. Transfus. Med., 2016, vol. 4, pp. 79–88. https://doi.org/10.2147/IJCTM.S101401

18. Lu, M., Lezzar, D.L., Vörös, E., and Shevkoplyas, S.S., Traditional and emerging technologies for washing and volume reducing blood products. J. Blood. Med., 2019, vol. 10, pp. 37–46. https://doi.org/10.2147/JBM.S166316