TSitologiya i Genetika 2020, vol. 54, no. 5, 21-26
Cytology and Genetics 2020, vol. 54, no. 5, 393–397, doi: https://www.doi.org/10.3103/S0095452720050047

Degradation of methanol catabolism enzymes of formaldehyde dehydrogenase and formate dehydrogenase in methylotrophic yeast Komagataella phaffii

Dmytruk О.V., Bulbotka N.V., Sibirny A.A.

  1. Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine
  2. Department of Biotechnology and Microbiology, University of Rzeszow, Zelwerowicza 4, Rzeszow 35-601 Poland

З’ясування механізмів деградації цитозольних білків має велике фундаментальне та прикладне значення. Досліджено зміни в активності цитозольних ферментів катаболізму метанолу – формальдегіддегідрогенази (Fldh1) та форміатдегідрогенази (Fdh1) у контрольного штаму дикого типу GS200, штаму з делецією гена сенсора гексоз GSS1 та штаму з дефектом автофагії SMD1163 K. phaffii при короткотривалій та довготривалій індукції метанолом з додаванням або ж без додавання інгібітора протеасомної деградації MG132. Показано, що тривалість інкубації клітин на метанолі не має особливого впливу на інактивацію ферменту. Ефект протеасомного інгібітора MG132 був незначний. Катаболітна інактивація цитозольних і пероксисомних ферментів пошкоджена в gss1∆ мутанта, оскільки пошкоджене сигналювання глюкозою. Fldh1 та Fdh1 ймовірно деградують вакуолярним шляхом, незалежно від тривалості індукції метанолом.

Keywords: formaldehyde dehydrogenase, formate dehydrogenase, specific activity, degradation, vacuoles, yeasts

TSitologiya i Genetika
2020, vol. 54, no. 5, 21-26

Current Issue
Cytology and Genetics
2020, vol. 54, no. 5, 393–397,
doi: 10.3103/S0095452720050047

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