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Structural pattern of CK1-like protein kinases associated with regulation of plant microtubule cytoskeleton

Karpov P.A., Rayevsky A.V., Sheremet Y.A., Yemets A.I., Blume Y.B.

SUMMARY. It was identified 18 isotypes of CK1-like protein kinases in A. thaliana. Comparison of catalytic domains in rat CK1 (α, β, γ_1-3, δ, and ε) and 18 plant homologs from A. thaliana, confirm high structural similarity for 13 CK1-like kinases: CKL1 (CK1δ), CKL2, CKL3, CKL4, CKL5, CKL6, CKL7, CKL8, CKL9, CKL10, CKL11, CKL12 and CKL13. It was found that CK1-specific inhibitor D4476 interact with rat KС1D and 13 plant homologes in similar ATP-competitive manner. Ligand binding were confirmed by docking scoring functions, molecular dynamics and chemogenomic analysis. The specific binding of CK1 kinases with substrate proteins depend on specific motifs located in their C-end region. The specific motifs of EB1 binding was identified in plant СKL1 and CKL2. Also, it was confirmed the role of C-end region in CKL6-β-tubulin interaction.

Key words: Casein kinase 1, plant homologues, tubulin, EB1, phosphorylation, inhibitor, D4476, Arabidopsis

Tsitologiya i Genetika 2020, vol. 54, no. 4, pp. 15-29

• Institute of Food Biotechnology and Genomics NAS of Ukraine, Ukraine 04123, Кyiv, Оsypovskoho str., 2а

E-mail: karpov nas.gov.ua

Karpov P.A., Rayevsky A.V., Sheremet Y.A., Yemets A.I., Blume Y.B. Structural pattern of CK1-like protein kinases associated with regulation of plant microtubule cytoskeleton, Tsitol Genet., 2020, vol. 54, no. 4, pp. 15-29.

In "Cytology and Genetics":
Karpov, A. V. Rayevsky, Ya. A. Sheremet, A. I. Yemets & Ya. B. Blume Structural Biological Characteristics of CK1-Like Protein Kinase Isotypes Associated with Regulation of Plant Microtubules, Cytol Genet., 2020, vol. 54, no. 4, pp. 293–304
DOI: 10.3103/S0095452720040052

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