TSitologiya i Genetika 2019, vol. 53, no. 6, 3-14
Cytology and Genetics 2019, vol. 53, no. 6, 441–450, doi: https://www.doi.org/10.3103/S0095452719060033

Arabidopsis root morphology and its correlation with cortical microtubule rearrangements caused by specific inhibition of protein kinases CK1

Karpov P.A., Sheremet Ya.A., Blume Ya.B., Yemets A.I.

SUMMARY. The members of the casein kinase 1 (CK1) subfamily are distinguished by strong interspecific conservatism and huge number of phosphorylation substrates. Recent data demonstrate evidence of direct CK1-dependent phosphorylation of tubulin. Consequently, CK1 protein kinases can contribute in the tubulin code and functional specialization of microtubules (MT). In this paper, we present new data on the plant tubulin cytoskeleton response provoked by treatment with D4476 – specific inhibitor of CK1. It was found that D4476 demonstrate a strong and dose-dependent effect on the growth and morphology of primary roots in Arabidopsis thaliana seed-lings. Subsequent experiments, on plants expressing a MT-associated fluorescent marker (GFP-Map4), have shown a pronounced correlation between morphological reac-tions and cortical rearrangements of microtubules caused by D4476-dependent inhibition of protein kinases CK1. 

Keywords: casein kinase 1, microtubules, phosphorylation, inhibitor, D4476, Arabidopsis

TSitologiya i Genetika
2019, vol. 53, no. 6, 3-14

Current Issue
Cytology and Genetics
2019, vol. 53, no. 6, 441–450,
doi: 10.3103/S0095452719060033

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