Molecular characterization and tissue expression of common tobacco (Nicotiana tabacum) cadmium resistance protein 2 and metal tolerance protein 4 genes

Kong C.S., Chen J.H., Liu J.H., Yu L.

Institute of Agricultural Environment&Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
Technology Center of Yunnan Tobacco Industry Co., Ltd., Kunming650231, China
Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, China

The complete coding sequences of common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were amplified by RTPCR. The tobacco cadmium resistance protein 2 gene encodes a protein of 192 amino acids which shares high identity with the cadmium resistance protein 2 of four species —nicotiana tomentosiformis (99 %), wood tobacco (97 %), capsicum annuum (90 %) and potato (86 %). The tobacco metal tolerance protein 4gene encodes a protein of 415 amino acids which shares high identity with themetal tolerance protein 4 of six species – nicotiana tomentosiformis (90 %), wood tobacco (90 %), lycopersicon esculentum (84 %), potato (84 %), lycopersicon pennellii (83 %) and capsicum annuum (82 %). Prediction of transmembrane helices showed that cadmium resistance protein 2 and metal tolerance protein 4 might be two transmembrane proteins. Phylogenetic analysis revealed that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes both have a closer genetic relationship with the wood tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes. Computerassisted analysis showed that cadmium resistance protein 2 gene is structured in 4 exons and 3 introns and metal tolerance protein 4 gene consists of 7 exons and 6 introns. The gene expression profile analysis indicated that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were highly expressed in root.

Keywords: Tobacco, molecular cloning, cadmium resistance protein 2, metal tolerance protein 4, tissue expression

TSitologiya i Genetika
2019, vol. 53, no. 2, 76-77

Cytology and Genetics
2019, vol. 53, no. 2, 169–177,
doi: 10.3103/S009545271902004X

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