TSitologiya i Genetika 2018, vol. 52, no. 6, 3-9
Cytology and Genetics 2018, vol. 52, no. 6, 395–399, doi: https://www.doi.org/10.3103/S0095452718060051

Accumulation of indole-3-acetic and abscisic acid in transformed roots of Artemisia vulgaris

Kosakivska I.V., Voytenko L.V., Drobot K.O., Matvieieva N.A.

SUMMARY. The character of accumulation and balance of endogenous indole-3-acetic and abscisic acids in Artemisia vulgaris roots transformed by Agrobacterium rhizogenes A4 was analyzed by high performance liquid chro-matography-mass spectrometry method. It was shown that the content of free IAA significantly exceeded the level of the conjugated form. The highest amount of active form of IAA (554,4 ± 27,7 ng/g of fresh weight) was recorded in line number 2. In roots of control samples dominated the free form of ABA. Its level was significantly higher than in the transformed lines. «Bearded» roots accumulated a conjugated form of ABA, the highest content of which in line number 4 reached 459,6 ± 23,0 ng/g of fresh weight. The architecture of the transformed roots was marked by significant branching, the formation of lateral roots; active accumulation of biomass is noted. The results showed changes in the balance of endogenous phytohormones in the «bearded» roots of A. vulgaris in the direction of domination of the free form of IAA in the context of a significant decrease of ABA.

SUMMARY. The character of accumulation and balance of endogenous indole-3-acetic and abscisic acids in Artemisia vulgaris roots transformed by Agrobacterium rhizogenes A4 was analyzed by high performance liquid chro-matography-mass spectrometry method. It was shown that the content of free IAA significantly exceeded the level of the conjugated form. The highest amount of active form of IAA (554,4 ± 27,7 ng/g of fresh weight) was recorded in line number 2. In roots of control samples dominated the free form of ABA. Its level was significantly higher than in the transformed lines. «Bearded» roots accumulated a conjugated form of ABA, the highest content of which in line number 4 reached 459,6 ± 23,0 ng/g of fresh weight. The architecture of the transformed roots was marked by significant branching, the formation of lateral roots; active accumulation of biomass is noted. The results showed changes in the balance of endogenous phytohormones in the «bearded» roots of A. vulgaris in the direction of domination of the free form of IAA in the context of a significant decrease of ABA.

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TSitologiya i Genetika
2018, vol. 52, no. 6, 3-9

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Cytology and Genetics
2018, vol. 52, no. 6, 395–399,
doi: 10.3103/S0095452718060051

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References

1. Munné-Bosch, S. and Müller, M., Hormonal cross-talk in plant development and stress responses, Front. Plant Sci., 2013, vol. 4, pp. 5–6. doi 10.3389/ fpls.2013.00529

2. Kolupaev, Yu.Ye. and Kosakivska, I.V., The role of signal systems and phytohormones in realization of plant stress response, Ukr. Bot. J., 2008, vol. 65, no. 3, pp. 418–430.

3. Park, Y.G., Mun, B.G., Kang, S.M., Hussain, A., Shahzad, R., Seo, C.W., Kim, A.Y., Lee, S.U., Oh, K.Y., Lee, D.Y., Lee, I.J., and Yun, B.W., Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones, PLoS One, 2017, vol. 12, no. 3. e0173203. doi 10.1371/journal.pone.0173203Central

4. Enders, T.A. and Strader, L.C., Auxin activity: past, present, and future, Am. J. Bot., 2015, vol. 102, no. 2, pp. 180–196. doi 10.3732/ajb.1400285Central

5. Spiess, G.M., Hausman, A., Yu, P., Cohen, J.D., Rampey, R.A., and Zolman, B.K., Auxin input pathway disruptions are mitigated by changes in auxin biosynthetic gene expression in Arabidopsis, Plant Physiol., 2014, vol. 165, pp. 1092–1104.Central

6. Endo, A., Okamoto, M., and Koshiba, T., ABA Biosynthetic and Catabolic Pathways, Dordrecht: Springer Science + Business Media, 2014.

7. Rowe, J.H., Topping, J.F., Liu, J., and Lindsey, K., Abscisic acid regulates root growth under osmotic stress conditions via an interacting hormonal network with cytokinin, ethylene and auxin, New Phytol., 2016, vol. 211, no. 1, pp. 225–239.Central

8. Gonzalez, A.A., Agbevenou, K., Herrbach, V., Gough, C., and Bensmihen, S., Abscisic acid promotes pre-emergence stages of lateral root development in Medicago truncatula, Plant Signal. Behav., 2015, vol. 10, no. 1. e977741. doi 10.4161/15592324.2014.977741

9. Luo, X., Chen, Z., Gao, J., and Gong, Z., Abscisic acid inhibits root growth in Arabidopsis through ethylene biosynthesis, Plant J., 2014, vol. 79, pp. 44–55.

10. Sakata, Y., Komatsu, K., and Takezawa, D., ABA as a universal plant hormone, Progr. Bot., 2014, vol. 75, pp. 57–96.

11. Drobot, K.O., Ostapchuk, A.M., Duplij, V.P., and Matvieieva, N.A., Effect of Agrobacterium rhizogenes-mediated transformation on the biologically active compounds content in Artemisia vulgaris L. transgenic roots, Plant Physiol. Genet., 2016, vol. 48, no. 5, pp. 550–555.

12. Sujatha, G., Zdravkovic-Korac, S., Calic, D., Flamini, G., and Ranjitha, KumariB.D., High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: Hairy root production and essential oil analysis, Industrial Crops Products, 2013, vol. 44, pp. 643–652. org/ doi 10.1016/j.indcrop.2012.09.007

13. Ali, M., Kiani, B.H., Mannan, A., Ismail, T., and Mirza, B., Enhanced production of artemisinin by hairy root cultures of Artemisia dubia, J. Med. Plants Res., 2012, vol. 6, no. 9, pp. 1619–1622. doi 10.5897/JMPR11.1268

14. Sparks, C.A., Doherty, A., and Jones, H.D., Genetic transformation of wheat via Agrobacterium-mediated DNA delivery, Meth. Mol. Biol., 2014, vol. 1099, pp. 235–250. doi 10.1007/978-1-62703-715-0_19

15. Kleinboelting, N., Huep, G., Appelhagen, I., Viehoever, P., Li, Y., and Weisshaar, B., The structural features of thousands of T-DNA insertion sites are consistent with a double-strand break repair-based insertion mechanism, Mol. Plant, 2015, vol. 8, no. 11, pp. 1651–1664. https://doi.org/10.1016/j.molp.2015.08.011

16. Boiko, G.V., Identification key for the species of the genus Artemisia L. (Asteraceae) of the flora Ukraine, Ukr. Bot. J., 2013, vol. 70, no. 4, pp. 479–481.

17. Correa-Ferreira, M.L., Verdan, M.H., and Reis Li-vero, F.A., Inulin-type fructan and infusion of Artemisia vulgaris protect the liver against carbon tetrachloride-induced liver injury, Phytomedicine, 2017, vol. 24, pp. 68–76.

18. Anwar, F., Ahmad, N., Alkharfy, K.M., and Gilani, A.-ul-H., Mugwort (Artemisia vulgaris) oils, in Essential Oils in Food Preservation, Flavor and Safety, Preedy, V.R., Ed., Elsevier, 2015, 1st ed., pp. 573–579.

19. Lian, G., Li, F., Yin, Y., Chen, L., and Yang, J., Herbal extract of Artemisia vulgaris (mugwort) induces antitumor effects in HCT-15 human colon cancer cells via autophagy induction, cell migration suppression and loss of mitochondrial membrane potential, J. Buon., 2018, vol. 23, no. 1, pp. 73–78.

20. Urban, J., Kokoska, L., Langrova, I., and Matejkova, J., In vitro anthelmintic effects of medicinal plants used in Czech Republic, Pharm. Biol., 2008, vol. 46, nos. 10–11, pp. 808–813.

21. Bamoniri, A., Mirjalili, B.B.F., Mazoochi, A., and Batooli, H., Chemical composition of Artemisia vulgaris L. from Kashan area isolated by nano scale injection, Iran. J. Org. Chem., 2010, vol. 2, no. 4, pp. 533–536.

22. Judzentiene, A. and Buzelyte, J., Chemical composition of essential oils of Artemisia vulgaris L. (mugwort) from North Lithuania, Chemija, 2006, vol. 17, no. 1, pp. 12–15.

23. Hristova, L., Damyanova, E., Doichinova, Z., and Kapchina-Toteva, V., Effect of 6-benzylaminopurine on micropropagation of Artemisia chamaemelifolia Vill. (Asteraceae), Bulg. J. Agricult. Sci., 2013, vol. 9, no 2, pp. 57–60.

24. Sujatha, G. and Ranjitha, KumariB.D., Effect of phytohormones on micropropagation of Artemisia vulgaris L., Acta Physiol. Plant., 2007, vol. 29, no. 3, pp. 189–195. doi 10.1007/s11738-006-0023-0

25. Sujatha, G. and Ranjitha, KumariB.D., Micropropagation, encapsulation and growth of Artemisia vulgaris node explants for germplasm preservation, South African J. Bot., 2008, vol. 74, no. 1, pp. 93–100.

26. Liu, C.Z., Murch, S.J., El-Demerdash, M., and Saxena, P.K., Regeneration of the Egyptian medicinal plant Artemisia judaica L., Plant Cell Rep., vol. 21, no. 6, pp. 525–530. doi 10.1007/s00299-002-0561-x

27. Rasool, R., Bashir, A.G., Kamili, A.N., Akbar, S., and Masood, A., Synergistic effect of auxins and cytokinins on propagation of Artemisia amygdalina (Asteraceae), a critically endangered plant of Kashmir, Pak. J. Bot., 2013, vol. 45, no. 2, pp. 629–634.

28. Zia, M., Riaz-ur-Rehman Chaudhary M.F., Hormonal regulation for callogenesis and organogenesis of Artemisia absinthium L., Afr. J. Biotech., 2007, vol. 6, no. 16, pp. 1874–1878.

29. Nilsson, O., Moritz, T., Imbault, N., Sandberg, G., and Olsson, O., Hormonal characterization of transgenic tobacco plants expressing the rolC gene of Agrobacterium rhizogenes TL-DNA, Plant Physiol., 1993, vol. 102, no. 2, pp. 363–371.Central

30. Pavlova, O.A., Matveyeva, T.V., and Lutova, L.A., Rol-genes of Agrobacterium rhizogenes, Ekol. Genet., 2013, vol. 11, no. 1, pp. 59–68.

31. Murashige, T. and Skoog, F., A revised medium for rapid growth and bio assay with tobacco tissue culture, Phys. Plant., 1962, vol. 15, pp. 473–497. org// doi 10.1111/j.1399-3054.1962.tb08052.x

32. Kosakivska, I.V., Voytenko, L.V., Likhnyovskiy, R.V., and Ustinova, A.Y., Effect of temperature on accumulation of abscisic acid and indole-3-acetic acid in Triticum aesticum L. seedling, Genet. Plant Physiol., 2014, vol. 4, nos. 3–4, pp. 201–208.

33. Leyser, O., Regulation of shoot branching by auxin, Trends Plant Sci., 2003, vol. 8, pp. 541–545. doi 10.1016/j.tplants.2003.09.008

34. Ludwig-Muller, J., Auxin conjugates: their role for plant development and in the evolution of land plants, J. Exp. Bot., 2011, vol. 62, no. 6, pp. 1757–1773. doi 10.1093/jxb/erq412

35. Piotrowska, A. and Bajguz, A., Conjugates of abscisic acid, brassinosteroids, ethylene, gibberellins and jasmonates, Phytochemistry, 2011, vol. 72, no. 17, pp. 2097–2112. doi 10.1016/j.phytochem.2011.08.012

36. Bishopp, A., Help, H., El-Showk, S., Weijers, D., Scheres, B., Friml, J., Benkova, E., Mahonen, A.P., and Helariutta, Y., A mutually inhibitory interaction between auxin and cytokinin specifies vascular pattern in roots, Curr. Biol., 2011, vol. 21, no. 11, pp. 917–926. doi 10.1016/j.cub.2011.04.017