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Effect of the 1BL.1RS wheat-rye translocation on the androgenic response in spring bread wheat

Lazaridou T.B., Pankou C.I., Xynias I.N., Roupakias D.G.

The study was conducted to investigate the effect of the 1BL.1RS wheat-rye translocation on the androgenic response in spring bread wheat. Therefore, four bread wheat cultivars carrying the translocation, four Greek and three Canadian bread wheat cultivars without the translocation were used. An equal number of anthers from each cultivar, containing microspores in the mid (MU) to late uninucleate (LU) microspore developmental stage, were cultured after cold pre-treatment for seven days at 4 ^ºC. W14, 190-2 and the basic MS were used as induction, regeneration, and rooting media respectively. The best androgenic response was recorded in two cultivars carrying the translocation. Only two cultivars lacking the translocation responded to anther culture. It is concluded that the positive effect of the 1BL.1RS translocation on anther culture response of bread wheat cultivars cannot be attributed entirely to its presence because the genetic background of the cultivars carrying the translocation could be also important.

Key words: anther culture, embryoid induction, green plant production

Tsitologiya i Genetika 2017, vol. 51, no. 6, pp. 83-85

• School of Agricultural Technology, Food Technology and Nutrition, Western Macedonia University of Applied Sciences, Terma Kontopoulou, 53100 Florina, Hellas, Greece
• Laboratory of Genetics and Plant breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Hellas, Greece

E-mail: ixynias teiwm.gr

Lazaridou T.B., Pankou C.I., Xynias I.N., Roupakias D.G. Effect of the 1BL.1RS wheat-rye translocation on the androgenic response in spring bread wheat, Tsitol Genet., 2017, vol. 51, no. 6, pp. 83-85.