TSitologiya i Genetika 2014, vol. 48, no. 3, 37-42
Cytology and Genetics 2014, vol. 48, no. 3, 166–170, doi: https://www.doi.org/10.3103/S0095452714030050

In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

Ghotbi Ravandi E., Rezanejad F., Dehghan E.

  • Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Iran
  • Department of Plant Biotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

Polyploidy has played a significant role in the evolutionary history of plants and is a valuable tool for obtaining useful characteristics. Because of the novelty of polyploids, comparison of their in vitro culture response with diploids would be notable. In this study, leaf explants from diploid, autotetraploid and mixoploid plants of Cichorium intybus L. were cultured in vitro on the similar media and under same conditions. The ploidy level of the obtained calluses and regenerants were determined by flow cytometry analysis. The callogenic response of leaf explants cultured on the callus induction medium did not depend on the ploidy level of their parental plants. According to the flow cytometry analysis, the increased ploidy levels (4x) and (8x) were observed in the callus cultures with diploid and tetraploid origin, respectively. A considerable difference was observed between the ploidy level of mixoploid plants and their calluses, indicating the dominance of diploid cells in the callus tissue. The results showed that polyploidy led to the loss of organogenic potential as the tetraploid origin calluses failed to regenerate, while the diploid origin calluses successfully regenerated to whole plants.

Keywords: Cichorium intybus, polyploidy, callus culture, organogenesis, flow cytometry.

TSitologiya i Genetika
2014, vol. 48, no. 3, 37-42

Current Issue
Cytology and Genetics
2014, vol. 48, no. 3, 166–170,
doi: 10.3103/S0095452714030050

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