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Induction of repair enzyme O⁶­methylguanine­DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro

Lylo V.V., Macewicz L.L., Kotsarenko K.V., Babenko L.A., Kornelyuk A.I., Suhorada E.M., Lukash L.L.

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The aim of our study was to investigate the effect of recombinant human cytokine EMAP II (endothelial monocyte-activating polypeptide II) on the expression of MGMT gene, encoding repair enzyme O⁶-methylguanine-DNA methyltransferase (MGMT) in human cell cultures. The influence of EMAP II on cell proliferation was performed using routine MTT assay. Identification of MGMT in cell extracts was performed using Western blot analysis. We used cell lines: A102 (fibroblasts), CB-1 (umbilical cord blood stromal cells), 4BL6 (cells derived from peripheral blood). It was shown that cytokine EMAP II caused induction of MGMT expression in studied human cell lines. There was a decrease in cell number at high concentrations of this cytokine. It was found that the presence of cytokine EMAP II in serum-free growth medium leads to increasing of repair enzyme MGMT expression level in human cells in vitro.

Tsitologiya i Genetika 2011, vol. 45, no. 6, pp. 53-60

E-mail: lukash imbg.org.ua, wbabenko_lesia ukr.n

Lylo V.V., Macewicz L.L., Kotsarenko K.V., Babenko L.A., Kornelyuk A.I., Suhorada E.M., Lukash L.L. Induction of repair enzyme O⁶­methylguanine­DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro, Tsitol Genet., 2011, vol. 45, no. 6, pp. 53-60.

In "Cytology and Genetics":
V. V. Lylo, L. L. Matsevich, E. V. Kotsarenko, L. A. Babenko, A. I. Kornelyuk, E. M. Sukhorada, L. L. Lukash Activation of gene expression of the O6-methylguanine-DNA-transferase repair enzyme upon the influence of EMAP II cytokine in human cells in vitro, Cytol Genet., 2011, vol. 45, no. 6, pp. 373-378
DOI: 10.3103/S0095452711060053