Gene therapy based on human mesenchymal stem cells : Strategies and methods

Major technologies of transient and stable transgene expression in hMSC are reviewed. Properties and efficiencies of recombinant lentiviruses, adenoviruses, AAV and baculoviruses used for hMSC transduction are compared. The aims of transgenesis include directed differentiation of hMSC, function improvement, correction of pathology factors and proliferatrion control, as well as many basic research challenges.

Keywords: mesenchymal stem cells (hMSC), transduction, gene therapy