Identification of allele variants of cattle milk productivity genes using pcr anti-primer method

In this work we have demonstrated two independent real-time PCR methods for detection single nucleotide polymorphism (SNP) of genes csn and acyl-CoA: diacylglycerol acyltransferase 1 (dgat) in cattle. We have analyzed 296 samples of milk production cattle of Ukrainian breeding. The genotype frequencies were АА – 0,58, АВ – 0,34, ВВ – 0,08 for csn gene and for dgat gene – АА – 0,7, АК – 0,26, КК – 0,04. High efficiency of so called «anti-primer» method was shown. Duration of anti-primer PCR reaction was about 2–2,5 hours only and provided full investigation of unknown gene allele.

Keywords: anti-primer PCR, SNP, CSN, DGAT1, detection of genes, cattle breed