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Fluorescence intensity profiles of in situ hybridization signals depict genome architecture within human interphase nuclei

Iourov I.Y., Vorsanova S.G., Yurov Y.B.

 


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An approach towards construction of two-dimensional (2D) and three-dimensional (3D) profiles of interphase chromatin architecture by quantification of fluorescence in situ hybridization (FISH) signal intensity is proposed. The technique was applied for analysis of signal intensity and distribution within interphase nuclei of somatic cells in different human tissues. Whole genomic DNA, fraction of repeated DNA sequences (Cot1) and cloned satellite DNA were used as probes for FISH. The 2D and 3D fluorescence intensity profiles were able to depict FISH signal associations and somatic chromosome pairing. Furthermore, it allowed the detection of replicating signal patterns, the assessment of hybridization efficiency, and comparative analysis of DNA content variation of specific heterochromatic chromosomal regions. The 3D fluorescence intensity profiles allowed the analysis of intensity gradient within the signal volume. An approach was found applicable for determination of assembly of different types of DNA sequences, including classical satellite and alphoid DNA, gene-rich (G-negative bands) and gene-poor (G-positive bands) chromosomal regions as well as for assessment of chromatin architecture and targeted DNA sequence distribution within interphase nuclei. We conclude the approach to be a powerful additional tool for analysis of interphase genome architecture and chromosome behavior in the nucleus of human somatic cells.

Tsitologiya i Genetika 2008, vol. 42, no. 5, pp. 3-8

  • National Research Center of Mental Health, Russian Academy of Medical Sciences, Moscow, Russia
  • Institute of Pediatrics and Children Surgery, Roszdrav, Moscow, Russia



Iourov I.Y., Vorsanova S.G., Yurov Y.B. Fluorescence intensity profiles of in situ hybridization signals depict genome architecture within human interphase nuclei, Tsitol Genet., 2008, vol. 42, no. 5, pp. 3-8.




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