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In vitro regeneration of Nicotiana africana plants in leaf and internode explants and from mesophyll protoplasts

Belokurova V.B., Golovach I.S., Shcherbak N.L., Kuchuk N.V.

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Methods of in vitro cultivation of an African endemic species Nicotiana africana (Solanaceae) possessing some valuable traits have been elaborated. Influence of different concentrations of auxin (a-naphtaleneacetic acid) and cytokinins (6-benzylaminopurine, zeatin, thidiazuron) on morphogenesis and plant regeneration has been analysed using leaves and internodes as explants. The optimum method of regeneration of N.africana shoots in leaf explants is cultivation in the presence of 0,1 mg/l NAA and 1 mg/l BA; in internode explants – cultivation on the medium containing 0,5 mg/l NAA and 1 mg/l zeatin. The use of thidiazuron was the most effective at the concentration of 0,4 mg/l with subsequent transfer of explants to hormone-free medium. Method of isolation and culture of mesophyll protoplasts enabling to regenerate N. africana plants during 3–4 months has been proposed.

Tsitologiya i Genetika 2004, vol. 38, no. 3, pp. 9-15

Belokurova V.B., Golovach I.S., Shcherbak N.L., Kuchuk N.V. In vitro regeneration of Nicotiana africana plants in leaf and internode explants and from mesophyll protoplasts, Tsitol Genet., 2004, vol. 38, no. 3, pp. 9-15.