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Application of DNA microelectrophoresis for estimation of genotoxic effects of mutagens in the culture of human haemopoietic cells

Chernenko V.Yu., Lukash L.L., Mazevich L.L., Kochubey T.P., Еlesiсhеv А.А, Klimenko E.A., Gorban L.N.

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SUMMARY. Formation of single- and double strand breakes in DNA which may be discovered by microelectrophoresis in agarose gel is one of the criterion of genetical lesions in cells as a result of apoptosis or of genotoxic agent action. Genotoxic action of nickel chloride at the level of DNA of the individual cells in the initial culture of human embryonic haemopoietic cells was studied. It has been shown that about 2% cells in the studied in vitro populations were in the apoptosis state. Nickel chloride induced increasing of the frequency of formation of electrophoretic tracks of «comet» type with destroyed DNA.

Tsitologiya i Genetika 2004, vol. 38, no. 1, pp. 31-35

Chernenko V.Yu., Lukash L.L., Mazevich L.L., Kochubey T.P., Еlesiсhеv А.А, Klimenko E.A., Gorban L.N. Application of DNA microelectrophoresis for estimation of genotoxic effects of mutagens in the culture of human haemopoietic cells, Tsitol Genet., 2004, vol. 38, no. 1, pp. 31-35.