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Inner and outer DNA loops in cell nuclei: evidence from pulsed-field comet assay

Chopei M., Olefirenko V., Afanasieva K., Sivolob A.


SUMMARY. At higher order levels chromatin is organized into loops, and this looping plays an important role in transcription regulation. In our previous works we investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) of nucleoids obtained from lysed cells. It was shown that there are three parts of DNA in nucleoids: DNA in rather small loops which migrate rapidly; DNA in the loops up to ~150 kb, the migration of which is retarded; and larger loops that cannot migrate. Here we applied, for the first time, the pulse-field electrophoresis in the comet assay. Our results show that the first rapid step during the usual comet assay can be attributed to loops on the nucleoid surface while the second slow component represents loops inside the nucleoid.

Tsitologiya i Genetika 2022, vol. 56, no. 4, pp. 3-9

  • Taras Shevchenko National University of Kyiv

E-mail: mariana.chopei, aphon

Chopei M., Olefirenko V., Afanasieva K., Sivolob A. Inner and outer DNA loops in cell nuclei: evidence from pulsed-field comet assay, Tsitol Genet., 2022, vol. 56, no. 4, pp. 3-9.

In "Cytology and Genetics":
M. Chopei, V. Olefirenko, K. Afanasieva & A. Sivolob Inner and Outer DNA Loops in Cell Nuclei: Evidence from Pulsed-Field Comet Assay, Cytol Genet., 2022, vol. 56, no. 4, pp. 313318
DOI: 10.3103/S0095452722040028


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